Determination of protein-drug binding constants by pressure-assisted capillary electrophoresis (PACE)/frontal analysis (FA).


A simple method for the determination of binding constants of drugs to human serum albumin (HSA) and alpha(1)-acid glycoprotein (AGP) was developed by pressured-assisted capillary electrophoresis (PACE) based on the principle of frontal analysis (FA). The free drug concentration was measured from the height of the frontal peak and calculated based on the… (More)