Determination of protein and RNA expression levels of common housekeeping genes in a mouse model of neurodegeneration.

@article{Calvo2008DeterminationOP,
  title={Determination of protein and RNA expression levels of common housekeeping genes in a mouse model of neurodegeneration.},
  author={Ana Cristina Calvo and Mar{\'i}a Moreno-Igoa and Raquel Manzano and Laura Ordov{\'a}s and Gema Yag{\"u}e and Sara Oliv{\'a}n and M Mu{\~n}oz and Pilar Zaragoza and Rosario Osta},
  journal={Proteomics},
  year={2008},
  volume={8 20},
  pages={4338-43}
}
The choice of housekeeping proteins or genes for internal standards should be made carefully, taking into account the cell and tissue type, the experimental conditions, and the healthy/disease state(s) under consideration. Furthermore, as the correlation between transcriptional and translational levels of commonly used housekeeping genes is often discussed, this study shed light on the transcriptional levels of beta-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the… CONTINUE READING
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b-Tubulin is a more suitable internal control than b-actin in Western blot analysis of spinal cord tissues after traumatic injury

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