Determination of protease cleavage site motifs using mixture-based oriented peptide libraries

@article{Turk2001DeterminationOP,
  title={Determination of protease cleavage site motifs using mixture-based oriented peptide libraries},
  author={Benjamin E. Turk and Lisa Li Fang Huang and Elizabeth T. Piro and Lewis C. Cantley},
  journal={Nature Biotechnology},
  year={2001},
  volume={19},
  pages={661-667}
}
The number of known proteases is increasing at a tremendous rate as a consequence of genome sequencing projects. Although one can guess at the functions of these novel enzymes by considering sequence homology to known proteases, there is a need for new tools to rapidly provide functional information on large numbers of proteins. We describe a method for determining the cleavage site specificity of proteolytic enzymes that involves pooled sequencing of peptide library mixtures. The method was… CONTINUE READING
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