Determination of potato carboxypeptidase inhibitor in African Green Monkey plasma using 96-well SPE and LC-MS/MS.

@article{Rose2005DeterminationOP,
  title={Determination of potato carboxypeptidase inhibitor in African Green Monkey plasma using 96-well SPE and LC-MS/MS.},
  author={Mark J. Rose and Carmen Fern{\'a}ndez-Metzler and B. A. Johns and Gary R. Sitko and Jacquelynn J. Cook and James Yergey},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2005},
  volume={38 4},
  pages={
          695-702
        }
}
Potato carboxypeptidase inhibitor (CPI), a peptide with multiple isoforms (MW>4000 Da) was determined from African Green Monkey plasma using a PE Sciex API-3000 LC-MS/MS in the positive ionization mode with the turbo ionspray interface (450 degrees C). Samples were prepared using an Oasis MCX 96-well solid phase extraction plate and chromatographed on an Allure C18 HPLC Column (50 mm x 1.0 mm, 5 microm) using gradient elution. Upon analysis of the extracts using LC-MS/MS, the concentration of… CONTINUE READING