Determination of plasmid molecular weights from ultraviolet sensitivities

  title={Determination of plasmid molecular weights from ultraviolet sensitivities},
  author={Aziz Sancar and Claud S. Rupert},
MECHANISMS for the repair of ultraviolet (200–300 nm) irradiated DNA have been well studied in connection with cells and bacteriophages1, but not with plasmids. During investigations of plasmid ultraviolet (UV) sensitivity by an Escherichia coli transformation assay2, we found that the principal E. coli repair systems act on UV-irradiated plasmid DNA, exerting a major effect on plasmid survival. In host cells lacking these repair functions, however, the UV survival of transforming activity… CONTINUE READING

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