Determination of plasma tissue factor antigen and its clinical significance.

  title={Determination of plasma tissue factor antigen and its clinical significance.},
  author={Tatsuya Koyama and Kazuhiro Nishida and Shinichi Ohdama and Michio Sawada and Naomi Murakami and Shinsaku Hirosawa and Renjiro Kuriyama and Katsuji Matsuzawa and Ryohei P. Hasegawa and Nobuo Aoki},
  journal={British journal of haematology},
  volume={87 2},
To investigate the clinical significance of determination of plasma tissue factor (TF) antigen, we have developed a highly sensitive enzyme-linked immunosorbent assay (ELISA) for plasma TF, using two different monoclonal antibodies against TF apoprotein, 6B4 (catching antibody) and 5G9 (detecting antibody), and tetramethyl benzidine/H2O2 as substrates. Titration curves of recombinant human TF in buffer containing Triton X-100 were linear within the range from 50 to 2000 pg/ml. The total assay… CONTINUE READING
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