Determination of plasma membrane lipid mass and composition in cultured Chinese hamster ovary cells using high gradient magnetic affinity chromatography.

@article{Warnock1993DeterminationOP,
  title={Determination of plasma membrane lipid mass and composition in cultured Chinese hamster ovary cells using high gradient magnetic affinity chromatography.},
  author={Dale E. Warnock and Christopher Digby Kinver Roberts and Manuela Lutz and Wendy Blackburn and William W. Young and Jacques U. Baenziger},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 14},
  pages={
          10145-53
        }
}
We have utilized wheat germ agglutinin conjugated to iron/dextran particles in conjunction with high gradient magnetic affinity chromatography (HIMAC) to prepare plasma membranes from cultured cells. Membrane-impermeable succinimidyl esters inactivate alkaline phosphodiesterase 1 (APDE-1) and were used to establish the proportion of APDE-1 expressed at the cell surface. The yield of inhibitable APDE-1 provides an accurate indication of plasma membrane yield, which was > 90% for Chinese hamster… CONTINUE READING

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SHOWING 1-10 OF 22 CITATIONS

PROBING LIPID DOMAINS IN MODEL MEMBRANES AND IN MAMMALIAN CELLS

VIEW 5 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

References

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Cell 6 3 , 1061 - 1 Oh i e s . Commun . 5 6 , 1010 - 1016

J. A. Tainer
  • Biochim . Biophys . Acta
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