Determination of plasma melatonin levels by enzyme-linked immunosorbent assay (EIA) in turbot (Scophtalmus maximus L.) and tench (Tinca tinca L.).

Abstract

An enzymoimmunoassay (EIA) kit for plasma melatonin (MLT) measurements was employed in tench (Tinca tinca) and in turbot (Scophtalmus maximus). Tench and turbot plasma samples were purified with a C18 reversed phase extraction columns because this kit is designed for human serum measurements. The lowest detection limit of the technique was 11.48 pg/well… (More)

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@article{Rebollar1999DeterminationOP, title={Determination of plasma melatonin levels by enzyme-linked immunosorbent assay (EIA) in turbot (Scophtalmus maximus L.) and tench (Tinca tinca L.).}, author={Pilar Garc{\'i}a Rebollar and E. Ubilla and Jos{\'e} Benito Peleteiro and Maria Teresa Agapito and Javier Alvari{\~n}o}, journal={Journal of physiology and biochemistry}, year={1999}, volume={55 4}, pages={341-7} }