Determination of pinocembrin in human plasma by solid-phase extraction and LC/MS/MS: application to pharmacokinetic studies.

@article{Yan2014DeterminationOP,
  title={Determination of pinocembrin in human plasma by solid-phase extraction and LC/MS/MS: application to pharmacokinetic studies.},
  author={Bei Yan and Guoying Cao and Taohua Sun and Xi Zhao and Xin Hu and Jiling Yan and Yueying Peng and Aixin Shi and Yang Li and Wei Xue and Min Li and Kexin Li and Yingfa Liu},
  journal={Biomedical chromatography : BMC},
  year={2014},
  volume={28 12},
  pages={1601-6}
}
A sensitive, fast and specific method for the quantitation of pinocembrin in human plasma based on high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) was developed and validated. Clonazepam was used as the internal standard (IS). After solid-phase extraction of 500 μL plasma, pinocembrin and the IS were separated on a Luna C8 column using the mobile phase composed of acetonitrile-0.3 mm ammonium acetate solution (65:35, v/v) at a flow rate of 0.25 mL/min in isocratic… CONTINUE READING