Determination of phosphorylated and O-glycosylated sites by chemical targeting (CTID) at ambient temperature.

@article{Hathaway2007DeterminationOP,
  title={Determination of phosphorylated and O-glycosylated sites by chemical targeting (CTID) at ambient temperature.},
  author={Gary M. Hathaway},
  journal={Methods in molecular biology},
  year={2007},
  volume={386},
  pages={79-93}
}
In the analytical approach called chemically targeted identification (CTID), peptides containing phosphorylated or glycosylated serine and threonine underwent beta-elimination to produce an unsaturated double bond. Nucleophilic addition of 2-aminoethanethiol to this bond occurred, yielding aminoethylcysteine. Thus, sites containing posttranslational modifications were made susceptible to lysine endopeptidase. Structural information could then be obtained by mass analysis of the proteolytic… CONTINUE READING