Determination of patulin by reversed-phase high-performance liquid chromatography with extraction by diphasic dialysis.

@article{Prieta1993DeterminationOP,
  title={Determination of patulin by reversed-phase high-performance liquid chromatography with extraction by diphasic dialysis.},
  author={J Prieta and M Andreina Moreno and J Bayo and Sandra D{\'i}az and G. Suarez and Laura Dominguez and Ramon Canela and Vicente Sanch{\'i}s},
  journal={The Analyst},
  year={1993},
  volume={118 2},
  pages={
          171-3
        }
}
A simple and economical method has been developed for the determination of patulin in apple juice. The sample is extracted with ethyl acetate in a diphasic dialysis system, and the extract is cleaned up by elution from a Sep-Pak cartridge. Patulin is detected and determined by reversed-phase high-performance liquid chromatography using a Novapak C18 column and an ultraviolet detector. The lower detection limit is 1 microgram l-1 and the recovery is 85% at the 20 micrograms l-1 level. 

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