Determination of nucleotide distances in RNA by means of copper phenanthroline-generated hydroxyl radical cleavage pattern.

@article{Hermann1995DeterminationON,
  title={Determination of nucleotide distances in RNA by means of copper phenanthroline-generated hydroxyl radical cleavage pattern.},
  author={Thomas Hermann and Hermann Heumann},
  journal={RNA},
  year={1995},
  volume={1 10},
  pages={1009-17}
}
In contrast to the commonly used Fe(II)-EDTA, bis(orthophenanthroline)-copper(I) (OP-Cu) first generates hydroxyl radicals after binding to RNA. Due to diffusion, the hydroxyl radicals can cleave neighboring nucleotides in a distance r of up to 1.5 nm to the OP-Cu binding site. Using the known structure of tRNAPhe as a reference, we show that the hydroxyl… CONTINUE READING