Determination of nonligand amino acids critical to [4Fe-4S]2+/+ assembly in ferredoxin maquettes.

  title={Determination of nonligand amino acids critical to [4Fe-4S]2+/+ assembly in ferredoxin maquettes.},
  author={Stephen E. Mulholland and Brian R. Gibney and F. P. {\'A}lvarez Rabanal and P Leslie Dutton},
  volume={38 32},
The prototype ferredoxin maquette, FdM, is a 16-amino acid peptide which efficiently incorporates a single [4Fe-4S]2+/+ cluster with spectroscopic and electrochemical properties that are typical of natural bacterial ferredoxins. Using this synthetic protein scaffold, we have investigated the role of the nonliganding amino acids in the assembly of the iron-sulfur cluster. In a stepwise fashion, we truncated FdM to a seven-amino acid peptide, FdM-7, which incorporates a cluster spectroscopically… CONTINUE READING


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