Determination of nicotine in tobacco with second-order spectra data of charge-transfer complex in ethanol-water binary solvents processed by parallel factor analysis.


A new spectrophotometric method for the determination of nicotine in mixtures without pre-separation has been proposed. Nicotine could react with 2,4-dinitrophenol through a charge-transfer reaction to form a colored complex. The second-order data from the visible absorption spectra of the complex in a series of ethanol-water binary solvents with various… (More)
DOI: 10.1016/j.saa.2010.02.013

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