Determination of motility forces of human spermatozoa using an 800 nm optical trap.

@article{Koenig1996DeterminationOM,
  title={Determination of motility forces of human spermatozoa using an 800 nm optical trap.},
  author={Karsten Koenig and Lars Othar Svaasand and Yuxiang Liu and Gregory J. Sonek and Pasquale Patrizio and Yona Tadir and Michael W. Berns and Bruce J. Tromberg},
  journal={Cellular and molecular biology},
  year={1996},
  volume={42 4},
  pages={501-9}
}
The measurement and calculation of trapping forces on ellipsoidal specimens, and the determination of intrinsic motility forces of human spermatozoa by employing an 800 nm optical trap ("laser tweezers"), are presented. ATP-driven motility forces were calculated from calibrated trapping forces generated during the interaction of an 800 nm laser beam with single sperm cells. Calibration was performed by moving optically trapped sperm heads through a laminar fluid and calculating viscous forces… CONTINUE READING

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