Determination of monoamine oxidase concentrations in rat liver by inhibitor binding.

@article{Gomez1986DeterminationOM,
  title={Determination of monoamine oxidase concentrations in rat liver by inhibitor binding.},
  author={Nidia Gomez and Mercedes Unzeta and Keith F. Tipton and Michael Anderson and Anne-Marie O'Carroll},
  journal={Biochemical pharmacology},
  year={1986},
  volume={35 24},
  pages={
          4467-72
        }
}
The concentrations of monoamine oxidase-A and -B have been determined in mitochondria, mitochondrial outer membranes and microsomes from Sprague-Dawley and Wistar rats by determining the binding of tritium-labelled pargyline. Although the amounts of each form present depended on the source and the preparation method, this was paralleled by the specific activity such that the molecular turnover number was found to remain constant. The catalytic constants, kcat/Km, which represents the apparent… CONTINUE READING
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