Determination of molecular stoichiometry without reference samples by analyzing fluorescence blinking with and without excitation synchronization.

  title={Determination of molecular stoichiometry without reference samples by analyzing fluorescence blinking with and without excitation synchronization.},
  author={Heike Hevekerl and Jerker Widengren},
  journal={Methods and applications in fluorescence},
  volume={3 2},
Stoichiometry of molecular complexes plays a crucial role in biology. Moreover, for quantitative fluorescence studies, it is often useful to know the number of fluorophores labeled onto the molecules studied. In this work, we propose an approach to determine the number of independent fluorescence emitters on fluorescent molecules based on fluorescence blinking caused by photo-induced triplet state formation, photo-isomerization or charge transfer. The fluorescence blinking is measured under two… 

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