Determination of methadone and its metabolites by high performance liquid chromatography following solid-phase extraction in rat plasma.

@article{Pierce1992DeterminationOM,
  title={Determination of methadone and its metabolites by high performance liquid chromatography following solid-phase extraction in rat plasma.},
  author={Timothy L. Pierce and Allan G. Murray and William Hope},
  journal={Journal of chromatographic science},
  year={1992},
  volume={30 11},
  pages={443-7}
}
This paper describes a rapid, quantitative liquid chromatographic analysis and extraction of methadone and its two major metabolites from rat plasma, using difenoxin as the internal standard. Using a C18 column, resolution of all sample components and the internal standard is achieved with a mobile phase of 25:75 acetonitrile-0.08% diethylamine in 1000 mL water, pH 2.3, at a flow rate of 1.5 mL/min. The injection volume is 100 microL. Standards are linear over the range 25-100 ng, with a lower… CONTINUE READING