Determination of melamine in pet food by enzyme immunoassay, high-performance liquid chromatography with diode array detection, and ultra-performance liquid chromatography with tandem mass spectrometry.

@article{Kim2008DeterminationOM,
  title={Determination of melamine in pet food by enzyme immunoassay, high-performance liquid chromatography with diode array detection, and ultra-performance liquid chromatography with tandem mass spectrometry.},
  author={Byungchul Kim and Lewis B Perkins and Rodney J. Bushway and Stephanie Nesbit and Titan Fan and Robert Sheridan and Virginia W. Greene},
  journal={Journal of AOAC International},
  year={2008},
  volume={91 2},
  pages={408-13}
}
Melamine in pet food (fortified or originally contaminated) was determined by enzyme immunoassay (EIA), high-performance liquid chromatography with diode array detection (HPLC-DAD), and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The limits of detection (LOD) for EIA and HPLC-DAD were 0.02 and 0.1 microg/mL, respectively. The linear ranges of the calibration curves for EIA and HPLC-DAD were 0.02-0.5 and 0.1-500 microg/mL, respectively. The coefficient of… CONTINUE READING
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