Determination of mannose and fructose in human plasma using deuterium labelling and gas chromatography/mass spectrometry.

@article{Pitknen1994DeterminationOM,
  title={Determination of mannose and fructose in human plasma using deuterium labelling and gas chromatography/mass spectrometry.},
  author={Esa Pitk{\"a}nen and Timo Kanninen},
  journal={Biological mass spectrometry},
  year={1994},
  volume={23 9},
  pages={590-5}
}
We used gas chromatography/mass spectrometry to measure mannose and fructose in human blood plasma. The plasma samples were treated with sodium borodeuteride. Characteristic ion fragments for (1-d)mannitol, (2-d)mannitol and (1-d,2-13C)mannitol were selected for use in sugar fragmentography and to build an algorithm for calculation of the sugar concentrations. The analytical recovery of mannose and fructose and the precision of the mannose assay were satisfactory. The measurement of fructose… CONTINUE READING