Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection.

@article{Mitrowska2005DeterminationOM,
  title={Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection.},
  author={Kamila Mitrowska and Andrzej Posyniak and Jan Żmudzki},
  journal={Journal of chromatography. A},
  year={2005},
  volume={1089 1-2},
  pages={187-92}
}
A liquid chromatography-VIS/FLD method for the analysis of malachite green (MG) and its major metabolite, leucomalachite green (LMG) in carp muscle has been described. The method consists in an extraction with acetonitrile-buffer mixture followed by partioning with dichloromethane. Clean up and isolation were performed on SCX solid phase extraction (SPE) column. Chromatographic separation was achieved by using phenyl-hexyl column with an isocratic mobile phase consisting of acetonitrile and… CONTINUE READING

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