Determination of lumefantrine in small-volume human plasma by LC-MS/MS: using a deuterated lumefantrine to overcome matrix effect and ionization saturation.

@article{Huang2012DeterminationOL,
  title={Determination of lumefantrine in small-volume human plasma by LC-MS/MS: using a deuterated lumefantrine to overcome matrix effect and ionization saturation.},
  author={Liusheng Huang and Xiaohua Li and Florence Marzan and Patricia S. Lizak and Francesca T. Aweeka},
  journal={Bioanalysis},
  year={2012},
  volume={4 2},
  pages={157-66}
}
BACKGROUND To support pediatric study, a method to determine lumefantrine (LF) with small sample volume is needed. Matrix effect (ME) is a daunting issue in LF quantification in human plasma with LC-MS/MS. RESULTS Here we report an LC-MS/MS method with a deuterated LF as the internal standard (IS). Plasma sample (25 µl) was acidified with 5% formic acid prior to extraction with ethyl acetate. The recovery was over 80%. The absolute ME was within the range of 100 ± 8% for both LF and the IS… CONTINUE READING

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