Determination of low levels of 2H-labeling using high-resolution mass spectrometry: application in studies of lipid flux and beyond.

@article{Herath2014DeterminationOL,
  title={Determination of low levels of 2H-labeling using high-resolution mass spectrometry: application in studies of lipid flux and beyond.},
  author={Kithsiri b. Herath and Wendy Zhong and Jiong Yang and Ablatt Mahsut and Rory J Rohm and Vinit Kirankumar Shah and Jos{\'e} Manuel Castro-P{\'e}rez and Haihong Zhou and Athula B Attygalle and Ling Zhi Kang and Shailendra B. Singh and Douglas Gerald Johns and Michele A. Cleary and Brian K. Hubbard and Stephen F. Previs and Thomas P. Roddy},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2014},
  volume={28 3},
  pages={
          239-44
        }
}
RATIONALE The ability to measure low levels of (2)H-labeling is important in studies of metabolic flux, e.g. one can estimate lipid synthesis by administering (2)H2O and then measuring the incorporation of (2)H into fatty acids. Unfortunately, the analyses are complicated by the presence of more abundant naturally occurring stable isotopes, e.g. (13)C. Conventional approaches rely on coupling gas chromatographic separation of lipids with either quadrupole-mass spectrometry (q-MS) and/or… CONTINUE READING
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