Determination of ligand-MurB interactions by isothermal denaturation: application as a secondary assay to complement high throughput screening.

@article{Sarver2002DeterminationOL,
  title={Determination of ligand-MurB interactions by isothermal denaturation: application as a secondary assay to complement high throughput screening.},
  author={Ronald W. Sarver and Joseph M. Rogers and Dennis E. Van Epps},
  journal={Journal of biomolecular screening},
  year={2002},
  volume={7 1},
  pages={
          21-8
        }
}
We used a temperature-jump isothermal denaturation procedure with various methods of detection to evaluate the quality of putative inhibitors of MurB discovered by high-throughput screening. Three optical methods of detection-ultraviolet hyperchromicity of absorbance, fluorescence of bound dyes, and circular dichroism-as well as differential scanning calorimetry were used to dissect the effects of two chemical compounds and a natural substrate on the enzyme. The kinetics of the denaturation… CONTINUE READING

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