Determination of leukotriene A4 stabilization by S100A8/A9 proteins using mass spectrometry

  title={Determination of leukotriene A4 stabilization by S100A8/A9 proteins using mass spectrometry},
  author={Christopher L. Rector and Robert C. Murphy},
  journal={Journal of Lipid Research},
  pages={2064 - 2071}
Leukotriene A4 (LTA4) is the precursor for the formation of bioactive leukotrienes, but is highly susceptible to nonenzymatic hydrolysis. Although it is chemically reactive, LTA4 participates in the process of transcellular metabolism, which requires the transfer of LTA4 from one cell to another for the production of additional leukotrienes. Due to the susceptibility of LTA4 to hydrolysis, various methods have been used to measure the half-life of LTA4 in the presence of different proteins in… Expand
Transcellular biosynthesis of eicosanoids.
The evidence for transcellular biosynthetic events, occurring in isolated cell preparations, complex isolated organ systems, and in vivo, that result in the production of prostaglandins, leukotrienes, and lipoxins are summarized. Expand
Lysophospholipid acyltransferases and leukotriene biosynthesis: intersection of the Lands cycle and the arachidonate PI cycle[S]
Leukotrienes (LTs) are autacoids derived from the precursor arachidonic acid (AA) via the action of five-lipoxygenase (5-LO) that are mediated by the disappearance of free AA from the nuclear membrane, transfer to the ER for Lands cycle reesterification into PI, and population of PI for cell membrane signaling. Expand
Functions of S100 proteins.
The S100 protein family consists of 24 members functionally distributed into three main subgroups: those that only exert intracellular regulatory effects, those with intracellular and extracellularExpand
Leukotrienes Are Dispensable for Vaginal Neutrophil Recruitment as Part of the Immunopathological Response During Experimental Vulvovaginal Candidiasis
Recruitment of polymorphonuclear neutrophils (PMNs) into the vaginal lumen is the hallmark of an acute immunopathologic inflammatory response during vulvovaginal candidiasis (VVC) caused by CandidaExpand


Stabilization of Leukotriene A4 by Epithelial Fatty Acid-binding Protein in the Rat Basophilic Leukemia Cell*
A novel function for the well studied fatty acid-binding protein as a participant in leukotriene biosynthesis that permits LTA4 to be available for further enzymatic processing in various cellular regions is presented. Expand
Transcellular metabolism of neutrophil-derived leukotriene A4 by human platelets. A potential cellular source of leukotriene C4.
The data indicate that platelets can produce large amounts of LTC4 from neutrophil-derived LTA4 and suggest that such interactions may occur in vivo and that platelet contribution could be an important contribution to the generation of the biologically active L TC4. Expand
Biosynthesis and metabolism of leukotrienes.
These metabolic transformations of the primary leukotrienes are critical for termination of their biological activity, and defects in expression of participating enzymes may be involved in specific genetic disease. Expand
Leukotriene C4 synthase
Site-directed mutagenesis of LTC 4 synthase suggests that Arg-51 is involved in opening the epoxide ring of LTA 4 and Tyr-93 in GSH thiolate anion formation during catalytic conjugation. Expand
Leukotriene A4 Hydrolase/Aminopeptidase, the Gatekeeper of Chemotactic Leukotriene B4 Biosynthesis*
This article gives an overview of the biochemical, structural, and catalytic properties of LTA4 hydrolase (LTA4H), which catalyzes the final and committed step in LTB4 biosynthesis. Expand
Fatty acid binding proteins stabilize leukotriene A4: competition with arachidonic acid but not other lipoxygenase products.
Five FABPs have now been examined and found to increase the half-life of LTA(4) at 4 degrees C to approximately 20 min with protein present and several lipoxygenase products, including 5-hydroxyeicosatetraenoic acid, and leukotriene B(4), were found to have no effect on the stability of L TA( 4) induced by E-FABP. Expand
Leukotriene A 4 Hydrolase / Aminopeptidase , the Gatekeeper of Chemotactic Leukotriene B 4 Biosynthesis *
The leukotrienes (LTs) are a family of lipid mediators that play important roles in a variety of allergic and inflammatory reactions (1, 2). These molecules are formed by leukocytes and are dividedExpand
Release of Leukotriene A4 Versus Leukotriene B4 from Human Polymorphonuclear Leukocytes*
The objective of this study was to determine the relative amount of leukotriene A4 synthesized by human polymorphonuclear leukocytes (PMNL) that is available for transcellular biosynthetic processes, and it was demonstrated that in human PMNL, the relative amounts of nonenzymatic versus enzymatic leuko A4-derived metabolites increased with decreasing cell concentrations. Expand
Leukotriene C(4) synthase.
  • B. Lam
  • Biology, Medicine
  • Prostaglandins, leukotrienes, and essential fatty acids
  • 1995
Site-directed mutagenesis of LTC(4) synthase suggests that Arg-51 is involved in opening the epoxide ring of LTA(4), and Tyr-93 in GSH thiolate anion formation during catalytic conjugation, suggesting significant homology to FLAP, mGST-2, and mGst-3. Expand
Enzyme with dual lipoxygenase activities catalyzes leukotriene A4 synthesis from arachidonic acid.
Several experiments indicated that both the 5-lipoxygenase and leukotriene A4 synthase activities resided in the same protein and that the formation of leuko A4 from 5-HPETE was catalyzed by the 8-lip Oxygenase activity of the enzyme. Expand