Determination of leukocyte DNA 6-thioguanine nucleotide levels by high-performance liquid chromatography with fluorescence detection.

Abstract

A HPLC method for determination of 6-thioguanine nucleotide in DNA was developed. Leukocyte DNA was isolated from peripheral blood, derivatized with chloroacetaldehyde and the formed etheno derivatives N(2),3-etheno 6-thioguanine (epsilon6TG), 1,N(6)-etheno adenine (epsilonA) and N(2),3-etheno guanine (epsilonG) were released from the DNA backbone by… (More)
DOI: 10.1016/j.jchromb.2008.02.007

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@article{Olesen2008DeterminationOL, title={Determination of leukocyte DNA 6-thioguanine nucleotide levels by high-performance liquid chromatography with fluorescence detection.}, author={K. -L. Olesen and Steen Honor{\'e} Hansen and Ulrik Sidenius and Kjeld Schmiegelow}, journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences}, year={2008}, volume={864 1-2}, pages={149-55} }