Determination of isoprene in human breath by thermal desorption gas chromatography with ultraviolet detection.


We describe a new, highly sensitive and specific method for the analysis of isoprene (2-methyl-1,3-butadiene) in human breath. A known volume of expired air (150 ml) was drawn through a solid sorbent material to capture trace organic substances, followed by thermal desorption at 200 degrees C and subsequent determination of isoprene by gas chromatography… (More)