Determination of isoniazid in human urine using screen-printed carbon electrode modified with poly-L-histidine.

Abstract

A sensitive voltammetric method for trace measurements of isoniazid (INZ) in synthetic human urine sample is described. The method is based on its electroreduction at -0.98V vs. Ag/AgCl on screen-printed carbon electrode (SPCE) modified with poly-l-histidine (PH). A film of good adherence on SPCE and electrocatalytic properties was obtained coating the electrode by histidine monomer electropolymerization process (SPCE/EPH). The electrochemical behavior of the modified SPCE was investigated by cyclic, linear sweep (LSV), differential pulse (DPV), square-wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS). Limits of detection of 5.0x10(-7) mol L(-1), 1.7x10(-7) mol L(-1) and 2.5x10(-7) mol L(-1) were estimated from LSV, DPV and SWV determinations, respectively. The method was successfully applied to the determination of INZ in human urine samples.

DOI: 10.1016/j.bioelechem.2009.07.010
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@article{Bergamini2010DeterminationOI, title={Determination of isoniazid in human urine using screen-printed carbon electrode modified with poly-L-histidine.}, author={M{\'a}rcio F. Bergamini and Daniela Pacheco dos Santos and Maria Valnice Boldrin Zanoni}, journal={Bioelectrochemistry}, year={2010}, volume={77 2}, pages={133-8} }