Determination of insulin in a single islet of Langerhans by high-performance liquid chromatography with fluorescence detection.

@article{Toriumi2002DeterminationOI,
  title={Determination of insulin in a single islet of Langerhans by high-performance liquid chromatography with fluorescence detection.},
  author={Chifuyu Toriumi and Kazuhiro Imai},
  journal={Analytical chemistry},
  year={2002},
  volume={74 10},
  pages={
          2321-7
        }
}
Rodents (rat and mouse) have two types of insulin (insulin I and II; each contains a universal chain A and a different composition of each type BI chain or type BII chain). The physiological role for each isomer is not yet clarified because of the lack of an appropriate separative determination method for these isomers. Thus, in this paper, a sensitive and selective HPLC-fluorescence determination method for the isomers was developed, which includes derivatization with a fluorogenic reagent for… CONTINUE READING