Determination of in vivo hepatic extraction ratio from in vitro metabolism by rat hepatocytes.

@article{Singh1991DeterminationOI,
  title={Determination of in vivo hepatic extraction ratio from in vitro metabolism by rat hepatocytes.},
  author={Kuldip Singh and Spencer L. Tripp and Alan W. Dunton and Frank L. Douglas and Ashok Rakhit},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={1991},
  volume={19 5},
  pages={990-6}
}
Using isolated rat hepatocytes, the in vitro intrinsic hepatic clearance of three drugs, namely, antipyrine (AP), propranolol (P), and CGS 13429 was determined. The hepatic extraction ratios of AP, P, and CGS 13429 were then calculated to be 0.10, 0.90, and 0.88, respectively. To determine the in vivo hepatic extraction ratio in the rat, AP, P, or CGS 13429 was infused separately at a rate of 40, 20, and 20 micrograms/min.kg, via jugular and hepatic portal veins. The steady state plasma levels… CONTINUE READING