Determination of immune complexes by high-performance gel chromatography (positive cooperativity of antibody-antigen reaction).

Abstract

Positive cooperativity of antibody-antigen complex formation using human Cu/Zn superoxide dismutase and a murine monoclonal antibody as model is demonstrated by high-performance gel chromatography using TSK G3000sw and TSK G4000sw columns. Three different antibody-antigen complexes named Type I, II and III composed from two antigen molecules+two antibody molecules, two antigen molecules+one antibody molecule and one antigen molecule+one antibody molecule could be separated. The degree and ratio of complexes present in solution depends mainly on the ratios of antibody and antigen.

Cite this paper

@article{Schroll1993DeterminationOI, title={Determination of immune complexes by high-performance gel chromatography (positive cooperativity of antibody-antigen reaction).}, author={Julian Schroll and Roman Necina and Chuck Tauer and Karola Vorauer and R Plot and Alois Jungbauer}, journal={Journal of biochemical and biophysical methods}, year={1993}, volume={27 2}, pages={117-26} }