Determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf by HPLC.

@article{Wang2003DeterminationOH,
  title={Determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf by HPLC.},
  author={Huafu Wang and Gordon J. Provan and Keith Helliwell},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2003},
  volume={33 4},
  pages={539-44}
}
An HPLC method for the determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf has been developed. The separation system consisted of a C18 reversed-phase column, a gradient elution system of methanol/water and orthophosphoric acid, and a photodiode array detector. The concentrations of hamamelitannin, gallic acid, (+)-gallocatechin and (+)-catechin were 4.77, 0.59, 0.22 and 0.39% (w/w), respectively, in the bark. Hamamelitannin and catechins were also… CONTINUE READING