Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release.

@article{Steward1999DeterminationOG,
  title={Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release.},
  author={Nancy S Steward and Roy O. Martin and Jean Marc Engasser and Jean Louis Goergen},
  journal={Biotechnology and bioengineering},
  year={1999},
  volume={66 2},
  pages={114-21}
}
The death of Medicago sativa L. cells cultivated in a batch culture was investigated by measuring both the appearance of intact dead cells determined on the basis of the trypan blue (TB) dye exclusion, and the release of the cytoplasmic esterase activity into the culture medium upon cell death. Taking into account the strong instability of this released esterase activity, the total dead cell and lysed cell densities have been estimated. A mechanism for cell death and lysis is proposed and the… CONTINUE READING

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A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
Whereas no lysis occurred during the first seven days , this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
Whereas no lysis occurred during the first seven days , this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
Whereas no lysis occurred during the first seven days , this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
Whereas no lysis occurred during the first seven days , this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
Whereas no lysis occurred during the first seven days , this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
The death of Medicago sativa L. cells cultivated in a batch culture was investigated by measuring both the appearance of intact dead cells determined on the basis of the trypan blue ( TB ) dye exclusion , and the release of the cytoplasmic esterase activity into the culture medium upon cell death .
The death of Medicago sativa L. cells cultivated in a batch culture was investigated by measuring both the appearance of intact dead cells determined on the basis of the trypan blue ( TB ) dye exclusion , and the release of the cytoplasmic esterase activity into the culture medium upon cell death .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
The death of Medicago sativa L. cells cultivated in a batch culture was investigated by measuring both the appearance of intact dead cells determined on the basis of the trypan blue ( TB ) dye exclusion , and the release of the cytoplasmic esterase activity into the culture medium upon cell death .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
Whereas no lysis occurred during the first seven days , this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
The death of Medicago sativa L. cells cultivated in a batch culture was investigated by measuring both the appearance of intact dead cells determined on the basis of the trypan blue ( TB ) dye exclusion , and the release of the cytoplasmic esterase activity into the culture medium upon cell death .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
Whereas no lysis occurred during the first seven days , this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
Whereas no lysis occurred during the first seven days , this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
Whereas no lysis occurred during the first seven days , this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
A mechanism for cell death and lysis is proposed and the specific rates of cell growth , death and lysis estimated .
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