Determination of glutathione and glutathione disulfide in biological samples.

@article{Anderson1985DeterminationOG,
  title={Determination of glutathione and glutathione disulfide in biological samples.},
  author={M. E. Anderson},
  journal={Methods in enzymology},
  year={1985},
  volume={113},
  pages={
          548-55
        }
}
  • M. Anderson
  • Published 1985
  • Chemistry, Medicine
  • Methods in enzymology
Publisher Summary There are a number of procedures, for example, chemical, enzymatic, and chromatographic for the determination of glutathione (GSH) and glutathione disulfide (GSSG) in biological samples. Enzymatic and chromatographic methods for the determination of glutathione in biological samples are described in this chapter. Because GSH readily oxidizes nonenzymatically and because it is a good substrate of γ-glutamyl transpeptidase, the biological samples are acidified quickly to reduce… Expand
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  • Chemistry, Medicine
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TLDR
It is reported here that 2-vinylpyridine is a much better reagent for the derivitization of glutathione, and it is demonstrated that the total glutATHione concentration in mouse plasma is substantially higher than generally reported and that glutathion disulfide constitutes less than 30% of the totalglutathione present. Expand
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TLDR
The use of the foregoing analytical method in the determination of total and oxidized glutathione contents of rat blood, kidney, and liver gave values in good agreement with those obtained by previous investigators. Expand
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TLDR
Oxidized glutathione content (average ± standard deviation) of human, rabbit, and rat erythrocytes has been shown to be 3.6 ± 1.6 mμmoles/ml RBC, and the GSSG content of rat liver, kidney, heart, lens, testes, and skeletal muscles is below the level of detection. Expand
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TLDR
The found that plasma allowed to stand at 23 degrees C for 30 to 60 min has total glutathione levels of 4 to 7 microM, most (95%) of which is GSSG; after treatment of this plasma (following deproteinization) with KBH4, levels of 21 to 24 microM were found, in accord with the view that GSH constitutes the major source of plasma thiol. Expand
High-performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfide, and related thiols and disulfides.
A rapid and sensitive high-performance liquid chromatography method for determination of nanomole levels of glutathione, glutathione disulfide, cysteine glutathione-mixed disulfide and 20 relatedExpand
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TLDR
These procedures have been tested extensively on kidney and are probably applicable to tissues in general. Expand
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TLDR
Since monobromobimane has little fluorescence and reacts very selectively with thiols to produce fluorescent derivatives, crude extracts can be derivatized and analyzed without prepurification of the thiolS, the entire process requiring only 1 to 2 h. Expand
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TLDR
Modifications of glutathione metabolism may be achieved by administration of selective enzyme inhibitors, and also by giving compounds that increase glutATHione synthesis, which are useful in chemotherapy and radiation therapy and in protecting cells against the toxic effects of drugs, other foreign compounds, and oxygen. Expand
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Abstract A method has been developed for the automated assay of glutathione and of related compounds utilizing an anion-exchange resin column in an amino acid analyzer. Reduced glutathione,Expand
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