Determination of glutamate-cysteine ligase (gamma-glutamylcysteine synthetase) activity by high-performance liquid chromatography and electrochemical detection.

@article{Gegg2002DeterminationOG,
  title={Determination of glutamate-cysteine ligase (gamma-glutamylcysteine synthetase) activity by high-performance liquid chromatography and electrochemical detection.},
  author={Matthew Gegg and John B. Clark and Simon J R Heales},
  journal={Analytical biochemistry},
  year={2002},
  volume={304 1},
  pages={
          26-32
        }
}
  • Matthew Gegg, John B. Clark, Simon J R Heales
  • Published in Analytical biochemistry 2002
  • Chemistry, Medicine
  • The tripeptide glutathione (gamma-glutamylcysteinylglycine; GSH) is the predominant low molecular mass thiol in cells. The function of GSH is of considerable interest, with the molecule being implicated in numerous cellular processes in addition to being a major cellular antioxidant. The enzyme glutamate-cysteine ligase (GCL) is the rate-limiting step in GSH synthesis. The GCL assay described here is based on high-performance liquid chromatography and exploits the electrochemically active… CONTINUE READING

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