Determination of glucosamine and N-acetyl glucosamine in fungal cell walls.

@article{Zamani2008DeterminationOG,
  title={Determination of glucosamine and N-acetyl glucosamine in fungal cell walls.},
  author={Akram Zamani and Azam Jeihanipour and Lars Edebo and Claes Niklasson and Mohammad J Taherzadeh},
  journal={Journal of agricultural and food chemistry},
  year={2008},
  volume={56 18},
  pages={8314-8}
}
A new method was developed to determine glucosamine (GlcN) and N-acetyl glucosamine (GlcNAc) in materials containing chitin and chitosan, such as fungal cell walls. It is based on two steps of hydrolysis with (i) concentrated sulfuric acid at low temperature and (ii) dilute sulfuric acid at high temperature, followed by one-step degradation with nitrous acid. In this process, chitin and chitosan are converted into anhydromannose and acetic acid. Anhydromannose represents the sum of GlcN and… CONTINUE READING

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