Determination of glucocerebroside, sphingomyelin, free fatty acid and total lipid by thin-layer chromatography and charring-scintillation quenching.

@article{Radin1982DeterminationOG,
  title={Determination of glucocerebroside, sphingomyelin, free fatty acid and total lipid by thin-layer chromatography and charring-scintillation quenching.},
  author={N. S. Radin and G. Deshmukh and Ramasamy Selvam and Ashok V. Hospattankar},
  journal={Biochimica et biophysica acta},
  year={1982},
  volume={713 2},
  pages={474-8}
}
A previously described method has been extended to various specific lipids of liver and brain. The basic method involves thin-layer chromatography followed by charring to reveal the bands. The intensity of each band is determined by suspending the silica gel in a radioactive scintillation gel and measuring the optically quenched activities. The lipids are… CONTINUE READING