Determination of global protein turnover in stressed mycobacterium cells using hybrid-linear ion trap-fourier transform mass spectrometry.

@article{Rao2008DeterminationOG,
  title={Determination of global protein turnover in stressed mycobacterium cells using hybrid-linear ion trap-fourier transform mass spectrometry.},
  author={Pragada Venkateswara Rao and Bryan A. P. Roxas and Qingbo Li},
  journal={Analytical chemistry},
  year={2008},
  volume={80 2},
  pages={
          396-406
        }
}
We determined the global protein turnover profiles for Mycobacterium smegmatis under acid shock and iron starvation conditions using a simple (15)N isotope doping technique and a complete medium replacement method for chasing. We used a high-resolution hybrid-linear ion trap-Fourier transform mass spectrometer coupled with nanoliquid chromatography separation to measure protein turnover values for 151 proteins over a dynamic range of 3 orders of magnitude ranging from about 0.2 to 500. Of these… CONTINUE READING
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