Determination of gentian violet, its demethylated metabolites, and leucogentian violet in chicken tissue by liquid chromatography with electrochemical detection.

@article{Roybal1990DeterminationOG,
  title={Determination of gentian violet, its demethylated metabolites, and leucogentian violet in chicken tissue by liquid chromatography with electrochemical detection.},
  author={Jos{\'e} E Roybal and Rosemary Munns and Jeffrey A Hurlbut and W Shimoda},
  journal={Journal - Association of Official Analytical Chemists},
  year={1990},
  volume={73 6},
  pages={940-6}
}
A method is presented for determination of residues of gentian violet (GV), its demethylated metabolites (pentamethyl and tetramethyl), and leucogentian violet (LGV) in chicken tissue. The analytes are extracted from tissue with acetonitrile/buffer and partitioned into methylene chloride. Polar lipids are removed on an alumina column followed by partitioning into methylene chloride from a citrate buffer. The compounds of interest are isolated on a disposable carboxylic acid cation exchange… CONTINUE READING