Determination of gentamicin in urine samples after inhalation by reversed-phase high-performance liquid chromatography using pre-column derivatisation with o-phthalaldehyde.

Abstract

Gentamicin and netilmicin (internal standard) were extracted from urine using C18 solid-phase extraction cartridges (94.3% recovery) and then derivatised with o-phthalaldehyde and 3-mercaptopropionic acid. The derivative was stable for >6 h. The mobile phase methanol-glacial acetic acid-water (800:20:180, v/v), contained 0.02 M sodium heptanesulfonic acid… (More)

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@article{AlAmoud2002DeterminationOG, title={Determination of gentamicin in urine samples after inhalation by reversed-phase high-performance liquid chromatography using pre-column derivatisation with o-phthalaldehyde.}, author={Abdulsalam I Al-Amoud and Brian J. Clark and Henry Chrystyn}, journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences}, year={2002}, volume={769 1}, pages={89-95} }