Determination of gemcitabine and its metabolite in human plasma using high-pressure liquid chromatography coupled with a diode array detector.

Abstract

AIM To establish a high-pressure liquid chromatography (HPLC) method for determination of the concentration of gemcitabine (dFdC) and its metabolite (dFdU) in human plasma. METHODS Plasma 1.0 mL spiked with floxuridine as an internal standard was extracted with 3.0 mL of methanol-acetonitrile (v/v, 1:9). The supernatant was evaporated at 60 centigrade and… (More)

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@article{Lin2004DeterminationOG, title={Determination of gemcitabine and its metabolite in human plasma using high-pressure liquid chromatography coupled with a diode array detector.}, author={Neng-ming Lin and Su Zeng and Sheng-lin Ma and Yunliu Fan and Hai-jun Zhong and Luo Fang}, journal={Acta pharmacologica Sinica}, year={2004}, volume={25 12}, pages={1584-9} }