Determination of folate in some cereals and commercial cereal-grain products consumed in Poland using trienzyme extraction and high-performance liquid chromatography methods

@article{Gujska2005DeterminationOF,
  title={Determination of folate in some cereals and commercial cereal-grain products consumed in Poland using trienzyme extraction and high-performance liquid chromatography methods},
  author={Elżbieta Gujska and Andrzej Kuncewicz},
  journal={European Food Research and Technology},
  year={2005},
  volume={221},
  pages={208-213}
}
A trienzyme treatment (rat plasma conjugase, α-amylase, protease) followed by affinity chromatography (with folate-binding protein from bovine milk) and reversed-phase high-performance liquid chromatography with UV and fluorescence detections was performed for quantification of different folate derivatives in some commercial cereal-grain products and cereals cultivated in Poland. The method described allowed good separation of the main folate vitamins. Of the commercial cereal-grain products… CONTINUE READING

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