Determination of flumequine and 7-hydroxyflumequine in plasma of sheep by high-performance liquid chromatography.

@article{Delmas1998DeterminationOF,
  title={Determination of flumequine and 7-hydroxyflumequine in plasma of sheep by high-performance liquid chromatography.},
  author={J M Delmas and Alain A Chapel and Pascal Sand{\'e}rs},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1998},
  volume={712 1-2},
  pages={263-8}
}
A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of flumequine and its metabolite 7-hydroxyflumequine in sheep plasma was described. The two compounds were extracted from 100 microl of plasma by liquid-liquid extraction. Aliquots (100 microl) were injected onto the HPLC system and separated on a LiChrospher Select B column with an isocratic system. The compounds were detected by fluorimetric detection for concentrations below 500 microg/l and by UV… CONTINUE READING