Determination of ergot alkaloids in feed by HPLC

@article{Ruhland2008DeterminationOE,
  title={Determination of ergot alkaloids in feed by HPLC},
  author={Monika Ruhland and Jeffery Tischler},
  journal={Mycotoxin Research},
  year={2008},
  volume={24},
  pages={73-79}
}
A HPLC method for the determination of ergometrine, ergotamine, ergocristine, α-ergocryptine and ergocornine in cereals for animal feed and in mixed feed with high cereal content was developed. Samples were extracted under acidic conditions using a mixture of phosphoric acid and acetonitrile, the extract purified with solid phase extraction cartridges (strong cation exchange), and ergot alkaloids detected after gradient elution on a C18 column by HPLC with fluorescence detection. Detection and… CONTINUE READING
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