Determination of endogenous ethanol in blood and breath by gas chromatography-mass spectrometry

  title={Determination of endogenous ethanol in blood and breath by gas chromatography-mass spectrometry},
  author={A. Wayne Jones and G{\"o}ran M{\aa}rdh and Erik E. {\"A}ngg{\aa}rd},
  journal={Pharmacology Biochemistry and Behavior},
Excretion of low-molecular weight volatile substances in human breath: focus on endogenous ethanol.
  • A. Jones
  • Medicine
    Journal of analytical toxicology
  • 1985
A gas chromatographic method suitable for the analysis of low-molecular endogenous volatiles in human breath is described, which found that when a man drank 5 g of exogenous ethanol, the blood concentration increased 200 times above the endogenous level.
Improvement of ethyl glucuronide determination in human urine and serum samples by solid-phase extraction.
  • I. Janda, A. Alt
  • Chemistry
    Journal of chromatography. B, Biomedical sciences and applications
  • 2001
Simultaneous determination of ethanol and methanol in alcohol free malt beverages, energy drinks and fruit juices by gas chromatography
A simple, sensitive, and direct method to decide if “alcohol-free” beverages, energy drinks and fruit juices could result in positive “alcohol alerts” based on the use of the gas chromatography with
A Simple Colorimetric Method for Determination of Ethanol in Exhaled Breath Condensate
A rapid and catalyst free colorimetric method with great potential for ethanol determination owing to many desirable properties such as high reliability, high sensitivity, and fast response time is developed.
Ethyl glucuronide excretion in humans following oral administration of and dermal exposure to ethanol.
Ethyl glucuronide (EtG) is a direct ethanol biomarker and U.S. Department of Health and Human Services has advised that specificity studies at low EtG levels are needed for distinction of ethanol
Urinary markers of chronic excessive ethanol consumption.
  • B. Tang
  • Medicine, Chemistry
    Alcoholism, clinical and experimental research
  • 1991
It was demonstrated that conjugates of ethanol and acetaldehyde were present in urine of alcoholics andUrinary levels of free ethanol and the conjugate in abstaining alcoholics were higher than those of social drinkers.
Effect of 4-methylpyrazole on endogenous plasma ethanol and methanol levels in humans.
The present study provides conclusive evidence for a constant endogenous production as well as clearance of ethanol and methanol in humans and shows that the ethanol and meethanol produced are, at least in part, eliminated by the ADH pathway.


Quantitative measurements of the alcohol concentration and the temperature of breath during a prolonged exhalation.
  • A. Jones
  • Medicine
    Acta physiologica Scandinavica
  • 1982
Results show that at least 70% of a man's vital capacity must be discarded before a breath-concentration plateau for ethanol develops, and even after a discard breath-volume of 10% FVC the concentration of alcohol reaches 80% of the level in end-expiratory breath.
Gas-chromatographic and mass-spectrometric detection of low-molecular-weight aliphatic alcohols in urine of normal individuals and patients with diabetes mellitus.
The aliphatic alcohols in 100 urines from 25 patients with diabetes mellitus under treatment with insulin, oral antidiabetic medication, or special diet are studied to show increased concentrations of ethanol, n-propanol, isobutanol, and isopentanol.
The physiological role of liver alcohol dehydrogenase.
The results indicate that significant amounts of alcohol are normally formed in the gastro-intestinal tract and the function, or a major function, of liver alcohol dehydrogenase is the detoxication of ethanol normally present.
The concentration of apparent endogenous ethanol.
  • D. Lester
  • Medicine
    Quarterly journal of studies on alcohol
  • 1962
Experiments have been performed in the manner suggested in the review in an attempt to clarify the issues, but it has not been possible to confirm the concentrations reported.
Endogenous ethanol: a review.
General agreement in the literature over the constant rate at which ingested ethanol is eliminated, the few opposing views notwithstanding, would seem to offer suppo rt for those who saw little real evidence of the endogenous occurrence of ethanol.
Methanol in Normal Human Breath
Free methanol has been detected and measured, by gas-liquid chromatography, in the breath of several normal, healthy humans. It is suggested that this is a metabolic product rather than the result of
Comparison of some porous polymers as adsorbents for collection of odour samples and the application of the technique to an environmental malodour
A range of Chromosorbs and Tenax-GC have been compared with regard to their efficiency as adsorbents for volatile odorous compounds using a very simple model system. When desorption was accomplished
Trace composition of human respiratory gas.
The use of the sampling technique to diagnose metabolic diseases and for application in the study of industrial exposure is reported on.