Determination of endocannabinoids in nematodes and human brain tissue by liquid chromatography electrospray ionization tandem mass spectrometry.

@article{Lehtonen2011DeterminationOE,
  title={Determination of endocannabinoids in nematodes and human brain tissue by liquid chromatography electrospray ionization tandem mass spectrometry.},
  author={M. Lehtonen and M. Storvik and H. Malinen and P. Hyyti{\"a} and M. Lakso and S. Auriola and G. Wong and J. Callaway},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2011},
  volume={879 11-12},
  pages={
          677-94
        }
}
  • M. Lehtonen, M. Storvik, +5 authors J. Callaway
  • Published 2011
  • Chemistry, Medicine
  • Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
A simple and highly sensitive liquid chromatography/tandem mass spectrometric (LC/MS/MS) method was developed to compare endogenous cannabinoid levels in nematodes and in brains of rats and humans, with and without prior exposure to ethanol. After liquid-liquid extraction of the lipid fraction from homogenized samples, a reversed-phase sub 2 μm column was used for separating analytes with an isocratic mobile phase. Deuterated internal standards were used in the analysis, and detection was made… Expand
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