Determination of clozapine and its metabolite, norclozapine in various biological matrices using high-performance liquid chromatography.

PURPOSE To develop and validate an HPLC method for the quantitation of clozapine and its metabolite, norclozapine in human plasma, rat plasma, and the various human plasma lipoprotein fractions. METHODS Using liquid-liquid extraction, clozapine, and norclozapine are extracted from the biological matrix with MTBE. After concentration, the residue was… (More)