Determination of cellular aminopropyltransferase activity using precolumn fluorescent etheno-derivatization with high-performance liquid chromatography.

@article{Yamazaki2012DeterminationOC,
  title={Determination of cellular aminopropyltransferase activity using precolumn fluorescent etheno-derivatization with high-performance liquid chromatography.},
  author={Ken-ichi Yamazaki and Yoshihiko Ikeguchi and Takuya Niwa and Kaoru Hayashi and Takahiro Iwaki and Ikumi Ishii and Masaru Niitsu and Anthony E. Pegg and A. Shirahata},
  journal={Analytical sciences : the international journal of the Japan Society for Analytical Chemistry},
  year={2012},
  volume={28 6},
  pages={
          621-4
        }
}
Polyamines such as spermidine (Spd) and spermine (Spm), produced by aminopropyltransferase (Apt), play roles in cell growth and differentiation. A sensitive and simple fluorometric high-performance liquid chromatographic determination for Apt activity of spermidine synthase (Spdsyn) and spermine synthase (Spmsyn) was developed in order to examine cellular functions of polyamine synthesis. The derivatization procedure for methylthioadenosine (MTA) produced from decarboxylated S… 
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1. VITAMIN B1 (THIAMINE MONONITRATE) 20% w/w 2. VITAMIN B2 (RIBOFLAVIN) 10% w/w 3. VITAMIN B3 (NICOTINAMIDE) 50% w/w 4. VITAMIN B5 (CALCIUM PANTOTHENATE) 10% w/w 5. VITAMIN B6 (PYRIDOXAL 5 PHOSPHATE)
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