Determination of ceftiofur and its metabolite desfuroylceftiofur in bovine serum and milk by ion-paired liquid chromatography.

Abstract

A simple and sensitive liquid chromatographic method has been developed for the simultaneous determination of ceftiofur and its metabolite desfuroylceftiofur in bovine serum and milk. The method involved an ultrafiltration of diluted serum/milk with an equal volume of 50% acetonitrile through a 10,000 dalton molecular mass cut-off filter. Separation of… (More)

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