Determination of biomass composition of Catharanthus roseus hairy roots for metabolic flux analysis.

@article{Sriram2006DeterminationOB,
  title={Determination of biomass composition of Catharanthus roseus hairy roots for metabolic flux analysis.},
  author={Ganesh Sriram and Omar Gonz{\'a}lez-Rivera and Jacqueline V. Shanks},
  journal={Biotechnology progress},
  year={2006},
  volume={22 6},
  pages={1659-63}
}
Metabolic flux analysis is a powerful diagnostic tool in metabolic engineering, and determination of biomass composition is indispensable to accurate flux evaluation. We report the elemental and biomolecular composition of Catharanthus roseus hairy roots, a pharmaceutically significant plant system and an important metabolic engineering target. The molecular formula of the organic material in the hairy roots was C12.0H22.7N0.4O7.6 during mid-exponential growth. The abundances of lipids, lignin… CONTINUE READING